碱性磷酸酶中单个氨基酸取代的免疫学检测。
Immunological detection of single amino acid substitutions in alkaline phosphatase.
作者信息
Cocks G T, Wilson A C
出版信息
Science. 1969 Apr 11;164(3876):188-9. doi: 10.1126/science.164.3876.188.
PMID:4886674
Abstract
Antiserums to wild-type alkaline phosphatase from Escherichia coli were prepared and tested for reactivity with phosphatases altered by point mutations. Eight out of the nine mutant enzymes were distinguished from the wild type with quantitative microcomplement fixation. The structural changes are among the smallest yet observed immunologically.
摘要
制备了针对大肠杆菌野生型碱性磷酸酶的抗血清，并检测其与因点突变而改变的磷酸酶的反应性。通过定量微量补体结合试验，从9种突变酶中区分出了8种与野生型不同的酶。这些结构变化是迄今为止在免疫学上观察到的最小变化之一。
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