肠杆菌科中的酶进化
Enzyme evolution in the Enterobacteriaceae.
作者信息
Cocks G T, Wilson A C
出版信息
J Bacteriol. 1972 Jun;110(3):793-802. doi: 10.1128/jb.110.3.793-802.1972.
Abstract
An immunological approach has been used for the study of alkaline phosphatase evolution in bacteria of the family Enterobacteriaceae. Antisera were prepared against alkaline phosphatase from Escherichia coli and Klebsiella aerogenes and tested against the unpurified alkaline phosphatases of 32 strains of enterobacteria by double diffusion and quantitative micro-complement fixation. The immunological relationships detected among the alkaline phosphatases of enterobacteria agree approximately with those reported for five other enzymes, as well as with the tryptic peptide pattern similarities found for two other enzymes, and with the relationships detected by interspecific deoxyribonucleic acid hybridization tests.
摘要
一种免疫学方法已被用于研究肠杆菌科细菌中碱性磷酸酶的进化。制备了针对大肠杆菌和产气克雷伯菌碱性磷酸酶的抗血清，并通过双向扩散和定量微量补体结合试验，对32株肠杆菌的未纯化碱性磷酸酶进行了检测。在肠杆菌碱性磷酸酶之间检测到的免疫关系，大致与其他五种酶所报道的关系相符，也与另外两种酶的胰蛋白酶肽图谱相似性相符，还与种间脱氧核糖核酸杂交试验所检测到的关系相符。
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