细菌脱氧核糖核酸聚合酶的血清学相关性
Serological relatedness of bacterial deoxyribonucleic acid polymerases.
作者信息
Tafler S W, Setlow P, Levine L
出版信息
J Bacteriol. 1973 Jan;113(1):18-23. doi: 10.1128/jb.113.1.18-23.1973.
Abstract
A number of bacterial species have been surveyed for serological activities with antiserum to Escherichia coli B deoxyribonucleic acid (DNA) polymerase I (EC 2.7.7.7.). The degree of serological cross-reaction is taken as a measure of relatedness of both the enzyme molecules from various species and the bacterial species themselves. Extracts were assayed by complement fixation only after treatment with deoxyribonuclease, since DNA bound to DNA polymerase alters the serological activity of the enzyme. Antiserum to E. coli DNA polymerase I did not react with either purified E. coli DNA polymerase II or the phage T4-induced DNA polymerase.
摘要
已针对多种细菌物种，用抗大肠杆菌B脱氧核糖核酸（DNA）聚合酶I（EC 2.7.7.7）的抗血清检测其血清学活性。血清学交叉反应的程度被用作衡量来自不同物种的酶分子以及细菌物种本身之间亲缘关系的指标。提取物仅在经过脱氧核糖核酸酶处理后，才通过补体结合试验进行检测，因为与DNA聚合酶结合的DNA会改变该酶的血清学活性。抗大肠杆菌DNA聚合酶I的抗血清与纯化的大肠杆菌DNA聚合酶II或噬菌体T4诱导的DNA聚合酶均无反应。
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