Auclair W, Siegel B W
Science. 1966 Nov 18;154(3751):913-5. doi: 10.1126/science.154.3751.913.
Late gastrulae of paracentrotus lividus regenerated cilia after being deciliated in hypertonic sea water. Regeneration was not affected by actinomycin D or puromycin. Actinomycin D also did not affect ciliary protein synthesis during regeneration, although overall embryonic synthesis was depressed. Puromycin inhibited both total embryonic and ciliary protein synthesis. The data indicate that ciliary protein synthesis is controlled by a stable template and that the regenerating cilia are formed from a pool of ciliary proteins. It is suggested that the proteins of the mitotic apparatus and of the ciliamay be related.
海胆的晚期原肠胚在高渗海水中去纤毛后能再生纤毛。再生不受放线菌素D或嘌呤霉素的影响。尽管整体胚胎合成受到抑制，但放线菌素D在再生过程中也不影响纤毛蛋白的合成。嘌呤霉素抑制整个胚胎和纤毛蛋白的合成。这些数据表明纤毛蛋白的合成受稳定模板的控制，并且再生的纤毛由纤毛蛋白库形成。有人提出有丝分裂器蛋白和纤毛蛋白可能有关。
