大肠杆菌K-12的硝酸还原酶复合体：无法还原硝酸盐的突变体的分离与特性分析
Nitrate reductase complex of Escherichia coli K-12: isolation and characterization of mutants unable to reduce nitrate.
作者信息
Ruiz-Herrera J, Showe M K, DeMoss J A
出版信息
J Bacteriol. 1969 Mar;97(3):1291-7. doi: 10.1128/jb.97.3.1291-1297.1969.
Abstract
Thirty-eight mutants unable to reduce nitrate were isolated from Escherichia coli and characterized biochemically and genetically. All of the mutants exhibited reduced or insignificant levels of formate dehydrogenase, nitrate reductase, or various combinations of these activities and cytochrome b(1) under conditions which resulted in the production of high levels of these activities by the wild-type parental strains. Most of the mutants reverted readily to wild type, and all mapped within a restricted region on the chromosome linked to the tryptophan genes. It was proposed that nitrate reduction in E. coli was catalyzed exclusively by an organized complex containing formate dehydrogenase, cytochrome b(1), and nitrate reductase.
摘要
从大肠杆菌中分离出38个无法还原硝酸盐的突变体，并对其进行了生化和遗传学特征分析。在野生型亲本菌株能产生高水平这些活性的条件下，所有突变体的甲酸脱氢酶、硝酸盐还原酶或这些活性与细胞色素b(1)的各种组合水平均降低或不显著。大多数突变体很容易回复为野生型，且所有突变体都定位在染色体上与色氨酸基因相连的一个受限区域内。有人提出，大肠杆菌中的硝酸盐还原仅由一种包含甲酸脱氢酶、细胞色素b(1)和硝酸盐还原酶的有序复合物催化。
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