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1
Nitrate reductase complex of Escherichia coli K-12: isolation and characterization of mutants unable to reduce nitrate.大肠杆菌K-12的硝酸还原酶复合体：无法还原硝酸盐的突变体的分离与特性分析
J Bacteriol. 1969 Mar;97(3):1291-7. doi: 10.1128/jb.97.3.1291-1297.1969.
2
Nitrate reductase complex of Escherichia coli K-12: participation of specific formate dehydrogenase and cytochrome b1 components in nitrate reduction.大肠杆菌K-12的硝酸还原酶复合体：特定甲酸脱氢酶和细胞色素b1组分在硝酸盐还原中的作用。
J Bacteriol. 1969 Sep;99(3):720-9. doi: 10.1128/jb.99.3.720-729.1969.
3
Phenotypic restoration by molybdate of nitrate reductase activity in chlD mutants of Escherichia coli.钼酸盐对大肠杆菌chlD突变体硝酸还原酶活性的表型恢复作用
J Bacteriol. 1971 Nov;108(2):854-60. doi: 10.1128/jb.108.2.854-860.1971.
4
Effects of molybdate and selenite on formate and nitrate metabolism in Escherichia coli.钼酸盐和亚硒酸盐对大肠杆菌中甲酸和硝酸盐代谢的影响。
J Bacteriol. 1971 Mar;105(3):1006-14. doi: 10.1128/jb.105.3.1006-1014.1971.
5
Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures.通过不同方法筛选的大肠杆菌硝酸还原酶突变体的比较。
Mol Gen Genet. 1972;116(1):1-10. doi: 10.1007/BF00334254.
6
Formation of the formate-nitrate electron transport pathway from inactive components in Escherichia coli.大肠杆菌中由无活性成分形成甲酸盐-硝酸盐电子传递途径。
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Defects in formate hydrogenlyase in nitrate-negative mutants of Escherichia coli.
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[Effect of nitrate reduction on carbon metabolism. 1. Changes in several enzyme activities in carbon metabolism in Escherichia coli by nitrate reduction].[硝酸盐还原对碳代谢的影响。1. 硝酸盐还原对大肠杆菌碳代谢中几种酶活性的影响]
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1
How FocA facilitates fermentation and respiration of formate by .FocA如何促进……对甲酸盐的发酵和呼吸作用。 （原文此处不完整，翻译可能存在一定局限性）
J Bacteriol. 2025 Feb 20;207(2):e0050224. doi: 10.1128/jb.00502-24. Epub 2025 Jan 27.
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Microbial Respiration and Formate Oxidation as Metabolic Signatures of Inflammation-Associated Dysbiosis.微生物呼吸与甲酸氧化作为炎症相关生态失调的代谢特征
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Genes, enzymes and membrane proteins of the nitrate respiration system ofEscherichia coli.大肠杆菌硝酸盐呼吸体系的基因、酶和膜蛋白。
J Membr Biol. 1972 Dec;9(1):195-207. doi: 10.1007/BF01868052.
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The hydrogenases and formate dehydrogenases of Escherichia coli.大肠杆菌的氢化酶和甲酸脱氢酶。
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Evidence of a second nitrate reductase activity that is distinct from the respiratory enzyme in Salmonella typhimurium.在鼠伤寒沙门氏菌中存在一种与呼吸酶不同的第二种硝酸还原酶活性的证据。
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Kinetic evaluation, using 13N, reveals two assimilatory nitrate transport systems in Klebsiella pneumoniae.利用¹³N进行的动力学评估揭示了肺炎克雷伯菌中的两种同化硝酸盐转运系统。
J Bacteriol. 1982 Jan;149(1):198-202. doi: 10.1128/jb.149.1.198-202.1982.
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9
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10
chlC (nar) operon of Escherichia coli includes structural genes for alpha and beta subunits of nitrate reductase.大肠杆菌的chlC（nar）操纵子包含硝酸还原酶α和β亚基的结构基因。
J Bacteriol. 1983 Mar;153(3):1513-20. doi: 10.1128/jb.153.3.1513-1520.1983.

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CHLORAMPHENICOL-PROMOTED REPRESSION OF beta-GALACTOSIDASE SYNTHESIS IN ESCHERICHIA COLI.氯霉素对大肠杆菌中β-半乳糖苷酶合成的促进性抑制作用
Proc Natl Acad Sci U S A. 1963 Mar;49(3):400-7. doi: 10.1073/pnas.49.3.400.
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BIOCHEMICAL ASPECTS OF GENETICS: THE OPERON.遗传学的生物化学层面：操纵子
Annu Rev Biochem. 1964;33:235-58. doi: 10.1146/annurev.bi.33.070164.001315.
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A colorimetric method for the assay of soluble succinic dehydrogenase and pyridinenucleotide-linked dehydrogenases.一种用于测定可溶性琥珀酸脱氢酶和吡啶核苷酸连接脱氢酶的比色法。
Arch Biochem Biophys. 1959 Dec;85:561-2. doi: 10.1016/0003-9861(59)90527-2.
4
[Mutations affecting the nitrate-reductase A and other bacterial enzymes of oxydoreduction. Preliminary study].[影响硝酸还原酶A及其他细菌氧化还原酶的突变。初步研究]
Ann Inst Pasteur (Paris). 1967 Jan;112(1):24-37.
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Alteration of respiratory particles by mutation in Escherichia coli K 12.大肠杆菌K12突变对呼吸颗粒的改变。
Biochem Biophys Res Commun. 1967 Apr 20;27(2):270-4. doi: 10.1016/s0006-291x(67)80073-1.
6
A gene cluster in Nuerospora crassa coding for an aggregate of five aromatic synthetic enzymes.粗糙脉孢菌中一个编码五种芳香族合成酶聚合体的基因簇。
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1453-60. doi: 10.1073/pnas.58.4.1453.
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Localization and regulation of synthesis of nitrate reductase in Escherichia coli.大肠杆菌中硝酸还原酶合成的定位与调控
J Bacteriol. 1968 Apr;95(4):1305-13. doi: 10.1128/jb.95.4.1305-1313.1968.
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The regulation of metabolism in facultative bacteria. 3. The effect of nitrate.兼性细菌中新陈代谢的调节。3. 硝酸盐的作用。
Biochim Biophys Acta. 1967 Oct 9;148(1):233-42. doi: 10.1016/0304-4165(67)90298-x.
9
Defects in formate hydrogenlyase in nitrate-negative mutants of Escherichia coli.
Biochem Biophys Res Commun. 1967 Sep 27;28(6):951-7. doi: 10.1016/0006-291x(67)90072-1.
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Enzymic properties of a mutant of Escherichia coli K12 lacking nitrate reductase.
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大肠杆菌K-12的硝酸还原酶复合体：无法还原硝酸盐的突变体的分离与特性分析

Nitrate reductase complex of Escherichia coli K-12: isolation and characterization of mutants unable to reduce nitrate.

作者信息

Ruiz-Herrera J, Showe M K, DeMoss J A

出版信息

J Bacteriol. 1969 Mar;97(3):1291-7. doi: 10.1128/jb.97.3.1291-1297.1969.

DOI:10.1128/jb.97.3.1291-1297.1969
PMID:4887509
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249846/
Abstract

Thirty-eight mutants unable to reduce nitrate were isolated from Escherichia coli and characterized biochemically and genetically. All of the mutants exhibited reduced or insignificant levels of formate dehydrogenase, nitrate reductase, or various combinations of these activities and cytochrome b(1) under conditions which resulted in the production of high levels of these activities by the wild-type parental strains. Most of the mutants reverted readily to wild type, and all mapped within a restricted region on the chromosome linked to the tryptophan genes. It was proposed that nitrate reduction in E. coli was catalyzed exclusively by an organized complex containing formate dehydrogenase, cytochrome b(1), and nitrate reductase.

摘要

从大肠杆菌中分离出38个无法还原硝酸盐的突变体，并对其进行了生化和遗传学特征分析。在野生型亲本菌株能产生高水平这些活性的条件下，所有突变体的甲酸脱氢酶、硝酸盐还原酶或这些活性与细胞色素b(1)的各种组合水平均降低或不显著。大多数突变体很容易回复为野生型，且所有突变体都定位在染色体上与色氨酸基因相连的一个受限区域内。有人提出，大肠杆菌中的硝酸盐还原仅由一种包含甲酸脱氢酶、细胞色素b(1)和硝酸盐还原酶的有序复合物催化。