通过羟基磷灰石色谱法从宿主脱氧核糖核酸中分离T-偶数噬菌体脱氧核糖核酸。
Separation of T-even bacteriophage deoxyribonucleic acid from host deoxyribonucleic acid by hydroxyapatite chromatography.
作者信息
Oishi M
出版信息
J Bacteriol. 1969 Apr;98(1):104-8. doi: 10.1128/jb.98.1.104-108.1969.
Abstract
A simple and rapid method is described for separation of T-even bacteriophage deoxyribonucleic acid (DNA) from host (Escherichia coli) DNA by hydroxyapatite column chromatography with a shallow gradient of phosphate buffer at neutral pH. By this method, bacteriophage T2, T4, and T6 DNA (but not T5, T7, or lambda DNA) could be separated from host E. coli DNA. It was found that glucosylation of the T-even phage DNA is an important factor in separation.
摘要
本文描述了一种简单快速的方法，可通过在中性pH下用磷酸盐缓冲液的浅梯度进行羟基磷灰石柱色谱，从宿主（大肠杆菌）DNA中分离T偶数噬菌体脱氧核糖核酸（DNA）。通过这种方法，可以从宿主大肠杆菌DNA中分离出噬菌体T2、T4和T6 DNA（但不能分离出T5、T7或λ DNA）。研究发现，T偶数噬菌体DNA的糖基化是分离的一个重要因素。
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本文引用的文献
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NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES.DNA分子短片段的核苷酸组成
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Protein chromatography on calcium phosphate columns.在磷酸钙柱上进行蛋白质色谱分析。
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Chromatography of nucleic acids on hydroxyapatite.核酸在羟基磷灰石上的色谱分析。
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