Tseng B Y, Marvin D A
J Virol. 1972 Sep;10(3):371-83. doi: 10.1128/JVI.10.3.371-383.1972.
Short pulses (30 sec at 32 C) of (3)H-thymidine were found primarily in the viral strands of replicating fd deoxyribonucleic acid (DNA), even at a time when most DNA being synthesized was duplex DNA. Much of the labeled viral strand DNA was longer than unit length, but some was shorter than unit length. Most of the corresponding complementary-strand DNA was recovered in closed supercoiled duplex molecules, even for short pulses; the remainder of the complementary-strand DNA was found in replicative intermediates in pieces shorter than unit length. Some of the viral strands in open replicating DNA lacked a corresponding complementary strand.
发现用(³H)胸腺嘧啶核苷进行的短脉冲(32℃下30秒)主要出现在正在复制的fd脱氧核糖核酸(DNA)的病毒链中,即使在大多数正在合成的DNA是双链DNA的时候也是如此。许多标记的病毒链DNA长度超过单位长度,但也有一些短于单位长度。即使是短脉冲,大部分相应的互补链DNA也在封闭的超螺旋双链分子中回收;互补链DNA的其余部分则在长度短于单位长度的复制中间体中发现。开放复制DNA中的一些病毒链缺乏相应的互补链。