Gharpure M A, Wright P F, Chanock R M
J Virol. 1969 Apr;3(4):414-21. doi: 10.1128/JVI.3.4.414-421.1969.
Four conditional-lethal temperature-sensitive mutants of RS virus were detected among the progeny of 454 plaques derived from virus grown in the presence of 10(-4)m 5-fluorouridine. These mutants were stable (reversion frequency, 10(-5.0) or less and failed to produce plaques at 38 or 39 C. Plaquing efficiency was depressed 100-fold or more at 37 C. Variable suppression of growth at the restrictive temperature of 39 C was observed, ranging from 16-fold to complete suppression. The temperature-sensitive defect of three of the mutants appeared to affect functions which were expressed late in the replicative cycle. One of the mutants produced atypical nonsyncytial plaques.
在由在10⁻⁴m 5-氟尿苷存在下培养的病毒产生的454个噬菌斑的子代中，检测到4株呼吸道合胞病毒的条件致死温度敏感突变体。这些突变体是稳定的（回复突变频率为10⁻⁵.⁰或更低），并且在38或39℃时不能产生噬菌斑。在37℃时，噬斑形成效率降低了100倍或更多。在39℃的限制温度下观察到生长的可变抑制，范围从16倍到完全抑制。其中3个突变体的温度敏感缺陷似乎影响了在复制周期后期表达的功能。其中一个突变体产生了非典型的非融合噬菌斑。
