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用对氟苯丙氨酸处理的大肠杆菌中的膜改变和异染粒的形成。

Membrane alteration and the formation of metachromatic granules in Escherichia coli treated with p-fluorophenylalanine.

作者信息

Brostrom M A, Binkley S B

出版信息

J Bacteriol. 1969 Jun;98(3):1263-70. doi: 10.1128/jb.98.3.1263-1270.1969.

DOI:10.1128/jb.98.3.1263-1270.1969
PMID:4892374
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC315322/
Abstract

The effect of p-fluorophenylalanine (FPA) on growing cultures of Escherichia coli was studied with regard to the composition and morphology of the cell envelope. A cell wall fraction was prepared by autolysis in hypertonic medium, and the resulting spheroplasts were osmotically lysed to obtain a cytoplasmic membrane fraction. Incorporation of labeled phenylalanine, FPA, and N-acetylglucosamine into both fractions of FPA-inhibited cells suggested that the composition of the membrane changed with time, whereas that of the cell wall remained relatively constant. Amino acid analysis revealed changes in the composition of the membrane fraction after FPA inhibition. Electron micrographs of shadowed cells and membranes revealed the presence of electron-dense metachromatic granules during the early stages of FPA inhibition.

摘要

研究了对氟苯丙氨酸（FPA）对大肠杆菌生长培养物的影响，涉及细胞包膜的组成和形态。通过在高渗培养基中自溶制备细胞壁组分，将所得原生质球进行渗透裂解以获得细胞质膜组分。将标记的苯丙氨酸、FPA和N-乙酰葡糖胺掺入FPA抑制细胞的两个组分中，表明膜的组成随时间变化，而细胞壁的组成保持相对恒定。氨基酸分析显示FPA抑制后膜组分的组成发生变化。对经投影的细胞和膜的电子显微照片显示，在FPA抑制的早期阶段存在电子致密的异染颗粒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/00055662b2da/jbacter00585-0450-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/a9dc5600f410/jbacter00585-0450-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/fa3bfe09eafa/jbacter00585-0450-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/872768eb65ae/jbacter00585-0450-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/c31c43ff4948/jbacter00585-0450-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/109f766432fb/jbacter00585-0450-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/00055662b2da/jbacter00585-0450-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/a9dc5600f410/jbacter00585-0450-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/fa3bfe09eafa/jbacter00585-0450-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/872768eb65ae/jbacter00585-0450-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/c31c43ff4948/jbacter00585-0450-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/109f766432fb/jbacter00585-0450-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9418/315322/00055662b2da/jbacter00585-0450-f.jpg

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引用本文的文献

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[Metabolic products of microorganisms. 88. Effect of L-2,5-dihydrophenylalanine--a phenylalanine antagonist].[微生物的代谢产物。88. L-2,5-二氢苯丙氨酸（一种苯丙氨酸拮抗剂）的作用]
Arch Mikrobiol. 1971;76(1):28-46.
2
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J Bacteriol. 1969 Jun;98(3):1271-3. doi: 10.1128/jb.98.3.1271-1273.1969.

本文引用的文献

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J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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EFFECTS OF P-FLUOROPHENYLALANINE ON LACTOSE-INDUCED BETA-GALACTOSIDASE SYNTHESIS IN RESTING-CELL SUSPENSIONS OF ESCHERICHIA COLI.对氟苯丙氨酸对大肠杆菌静止细胞悬液中乳糖诱导的β-半乳糖苷酶合成的影响
Arch Biochem Biophys. 1964 Nov;108:314-22. doi: 10.1016/0003-9861(64)90392-3.
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SLOW EXPONENTIAL GROWTH OF ESCHERICHIA COLI IN PRESENCE OF RHO-FLUOROPHENYLALANINE. EFFECT OF THE ANALOG ON AROMATIC BIOSYNTHESIS.在存在 Rho-氟苯丙氨酸的情况下大肠杆菌的缓慢指数生长。该类似物对芳香族生物合成的影响。
Biochim Biophys Acta. 1964 Jun 22;87:277-90. doi: 10.1016/0926-6550(64)90223-3.
5
DE NOVO SYNTHESIS OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS.嗜热脂肪芽孢杆菌对α-淀粉酶的从头合成
J Bacteriol. 1963 Dec;86(6):1202-10. doi: 10.1128/jb.86.6.1202-1210.1963.
6
INORGANIC POLYPHOSPHATE METABOLISM IN CHLOROBIUM THIOSULFATOPHILUM.嗜硫代硫酸盐绿菌中的无机多磷酸盐代谢
J Bacteriol. 1963 Mar;85(3):577-84. doi: 10.1128/jb.85.3.577-584.1963.
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Studies on the mechanism of protein synthesis; incorporation of p-fluorophenylalanine into alpha-amylase of Bacillus subtilis.蛋白质合成机制的研究；对氟苯丙氨酸掺入枯草芽孢杆菌α淀粉酶的研究。
Biochim Biophys Acta. 1960 Jun 17;41:98-103. doi: 10.1016/0006-3002(60)90373-5.
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Amino acid analog incorporation into bacterial proteins.氨基酸类似物掺入细菌蛋白质中。
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Immunological properties of exopenicillinase synthesized by Bacillus cereus 569/H in the presence of amino acid analogues.蜡样芽孢杆菌569/H在氨基酸类似物存在下合成的外青霉素酶的免疫学特性。
Biochem J. 1960 Oct;77(1):112-21. doi: 10.1042/bj0770112.
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A modified procedure for the automatic analysis of amino acids.一种用于氨基酸自动分析的改进方法。
Anal Biochem. 1960 Nov;1:187-201. doi: 10.1016/0003-2697(60)90045-2.