Brostrom M A, Binkley S B
J Bacteriol. 1969 Jun;98(3):1271-3. doi: 10.1128/jb.98.3.1271-1273.1969.
Treatment of exponentially growing Escherichia coli cells with the amino acid analogue, p-fluorophenylalanine (FPA), resulted in a decrease in the amount of deoxyribonucleic acid (DNA) present per cell. A minimum DNA content per cell was reached approximately 80 min after addition of FPA to the culture. After this time the DNA per cell rose to the level of the untreated cell. When an FPA-treated culture was resuspended in fresh medium containing no FPA 80 min after addition of the analogue, the increases in cell number and DNA became synchronous. Ribonucleic acid was synthesized immediately and continuously throughout the cell cycle, except during periods of DNA synthesis and cell division.
用氨基酸类似物对羟基苯丙氨酸（FPA）处理指数生长的大肠杆菌细胞，导致每个细胞中脱氧核糖核酸（DNA）的含量减少。在向培养物中添加FPA后约80分钟，达到每个细胞的最低DNA含量。此后，每个细胞的DNA上升到未处理细胞的水平。当在添加类似物80分钟后将FPA处理的培养物重悬于不含FPA的新鲜培养基中时，细胞数量和DNA的增加变得同步。核糖核酸在整个细胞周期中立即且持续合成，除了DNA合成和细胞分裂期间。
