Kanoh S, Kohlhage H, Siegert R
J Bacteriol. 1968 Sep;96(3):738-43. doi: 10.1128/jb.96.3.738-743.1968.
Experiments were performed to characterize the pyrogenic principle of ribonucleic acid (RNA) from the yeast Candida utilis. It was shown that ribonuclease hydrolysis of the RNA does not lead to inactivation of the pyrogenicity. Pyrogenicity was, however, destroyed by treatment with sodium deoxycholate. On column chromatography with Biogel under sterile and pyrogen-free conditions, the pyrogenic principle of yeast RNA was eluted together with the RNA. After treatment of the RNA with ribonuclease, it was possible to separate the pyrogenic activity from the RNA (hydrolysis products) to a great extent. Column chromatography of Escherichia coli endotoxin showed that the endotoxin was eluted in the same fractions as the pyrogenic activity of yeast RNA. On the basis of the behavior of the pyrogen, it may very well be that the fever reaction is produced not by the nucleic acid but by pyrogenic contaminants of the RNA preparation.
进行了实验以表征产朊假丝酵母核糖核酸（RNA）的致热原理。结果表明，RNA经核糖核酸酶水解不会导致致热活性丧失。然而，用脱氧胆酸钠处理可破坏其致热活性。在无菌和无热原条件下用Biogel进行柱色谱分析时，酵母RNA的致热原理与RNA一起被洗脱。用核糖核酸酶处理RNA后，可在很大程度上从RNA（水解产物）中分离出致热活性。大肠杆菌内毒素的柱色谱分析表明，内毒素与酵母RNA的致热活性在相同的馏分中被洗脱。基于该致热原的行为，很可能发热反应不是由核酸产生的，而是由RNA制剂的致热污染物产生的。
