Guthrie C, Nashimoto H, Nomura M
Proc Natl Acad Sci U S A. 1969 Jun;63(2):384-91. doi: 10.1073/pnas.63.2.384.
E. coli mutants that fail to grow at 20 degrees C were isolated, and three such mutants were studied in detail. Two of these are defective in 50S ribosome biosynthesis at low temperatures and accumulate 43S and 32S particles, respectively. The third mutant produces reduced amounts of 50S ribosomes and accumulates 21S particles at low temperatures. The 43S and 32S particles contain 23S RNA and appear to be precursors of 50S ribosomes. The 21S particles contain 16S RNA. The mutation that causes accumulation of 32S particles is linked to the spc locus. Isolation and characterization of coldsensitive mutants should provide a fruitful approach to the study of in vivo ribosomal assembly.
分离出了在20摄氏度下无法生长的大肠杆菌突变体，并对其中三个这样的突变体进行了详细研究。其中两个在低温下50S核糖体生物合成存在缺陷，分别积累43S和32S颗粒。第三个突变体在低温下产生的50S核糖体数量减少，并积累21S颗粒。43S和32S颗粒含有23S RNA，似乎是50S核糖体的前体。21S颗粒含有16S RNA。导致32S颗粒积累的突变与spc位点相关。对冷敏感突变体的分离和表征应为体内核糖体组装的研究提供一种富有成效的方法。
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