细菌原生质中的隐匿内毒素。
Occult endotoxin in bacterial protoplasm.
作者信息
Rudbach J A, Milner K C, Ribi E
出版信息
J Bacteriol. 1969 Jul;99(1):51-6. doi: 10.1128/jb.99.1.51-56.1969.
Abstract
Protoplasm separated from disrupted cells of gram-negative bacteria was extracted with hot phenol-water or was precipitated with ethyl alcohol after digestion with Pronase. These methods recovered about 10 times more endotoxin than was detectable in the untreated protoplasm. Inactivation of endotoxin by protoplasm also occurred in vitro when the endotoxin was first dissociated into subunits before reaction with protoplasm. Despite this increased yield from another source, the major proportion of endotoxin was still found in the cell walls.
摘要
从革兰氏阴性菌破碎细胞中分离出的原生质体，用热酚 - 水提取，或在用链霉蛋白酶消化后用乙醇沉淀。这些方法回收的内毒素比未处理的原生质体中可检测到的内毒素多约10倍。当内毒素在与原生质体反应之前先解离成亚基时，原生质体对内毒素的体外失活也会发生。尽管从另一个来源获得了更高的产量，但大部分内毒素仍存在于细胞壁中。
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本文引用的文献
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