Tarmina D F, Milner K C, Ribi E, Rudbach J A
J Bacteriol. 1968 Nov;96(5):1611-6. doi: 10.1128/jb.96.5.1611-1616.1968.
Endotoxin dissociated into subunits by sodium deoxycholate treatment exhibited diminished capacity to kill chick embryos, protect mice against the lethal effects of infection with Salmonella typhi, evoke hemorrhagic necrosis in skin inoculated with epinephrine, prepare for and provoke the dermal Shwartzman reaction, and induce pyrogenic tolerance. Surfactant-treated material which had been allowed to reaggregate displayed activity equivalent to that of untreated material. These findings were consistent with the working hypothesis that a macromolecular complex of critical size is required in order for endotoxin to elicit its characteristic effects in the host.
经脱氧胆酸钠处理后解离成亚基的内毒素，其杀死鸡胚的能力减弱，保护小鼠抵抗伤寒沙门氏菌感染致死效应的能力减弱，在用肾上腺素接种的皮肤中引发出血性坏死的能力减弱，引发和诱发皮肤施瓦茨曼反应的能力减弱，以及诱导热原耐受的能力减弱。经表面活性剂处理后再聚集的物质表现出与未处理物质相当的活性。这些发现与工作假设一致，即内毒素要在宿主体内引发其特征性效应，需要一个临界大小的大分子复合物。
