Smith H S, Pizer L I, Pylkas L, Lederberg S
J Virol. 1969 Aug;4(2):162-8. doi: 10.1128/JVI.4.2.162-168.1969.
We have investigated some of the biochemical events that accompany the abortive infection by T2 of Shigella dysenteriae lysogenized with the temperate phage P2. After infection with T2, protein and RNA synthesis continued for 3 to 5 min. The virus-induced enzyme, deoxycytidylate hydroxymethylase was produced in reduced amounts (15% of normal), and the extent of deoxyribonucleic acid (DNA) synthesis was 0.1% of that found with a nonlysogenic strain. Measurements of the production of acid-soluble fragments and sedimentation analyses failed to detect enzymatic degradation of the infecting viral DNA which could be specifically related to the presence of the prophage P2. Each interaction between T2 and a bacterium resulted in the death of the cell. This observation is consistent with results obtained with other types of bacteria which show that only when a nucleolytic attack occurs on T2 DNA does the cell have an increased capacity to survive after adsorption of T2.
我们研究了一些伴随着温和噬菌体P2溶源化的痢疾志贺氏菌被T2噬菌体流产感染的生化事件。用T2噬菌体感染后，蛋白质和RNA合成持续3至5分钟。病毒诱导的酶——脱氧胞苷酸羟甲基化酶的产生量减少（为正常量的15%），脱氧核糖核酸（DNA）合成程度为非溶源菌株的0.1%。对酸溶性片段产生的测量和沉降分析未能检测到与原噬菌体P2存在相关的感染性病毒DNA的酶促降解。T2与细菌之间的每次相互作用都会导致细胞死亡。这一观察结果与用其他类型细菌获得的结果一致，这些结果表明只有当T2 DNA发生核酸酶攻击时，细胞在吸附T2后才有增加的存活能力。
