链球菌抗脱氧核糖核酸酶B的微量试验
Micro test for streptococcal anti-deoxyribonuclease B.
作者信息
Klein G C, Baker C N, Addison B V, Moody M D
出版信息
Appl Microbiol. 1969 Aug;18(2):204-6. doi: 10.1128/am.18.2.204-206.1969.
Abstract
A methyl green decolorization micro test for the determination of streptococcal anti-deoxyribonuclease B titers in serum is described. The micro test was compared with an alcohol precipitation macro test on 112 human sera. The anti-deoxyribonuclease B titers agreed within one dilution step for 85.7% of the 112 sera examined by both techniques. Both tests were about equal in the detection of titers above the upper limits of normal. The micro test requires smaller amounts of reagents and fewer man-hours to perform than the macro test. This translates into lower cost per test and more tests per man-hour.
摘要
本文描述了一种用于测定血清中链球菌抗脱氧核糖核酸酶B滴度的甲基绿脱色微量试验。将该微量试验与112份人血清的酒精沉淀常量试验进行了比较。两种技术检测的112份血清中，有85.7%的血清抗脱氧核糖核酸酶B滴度在一个稀释度范围内相符。在检测高于正常上限的滴度方面，两种试验大致相当。与常量试验相比，微量试验所需的试剂较少，操作所需工时也较少。这意味着每次试验成本更低，每人时可进行的试验更多。
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