Moody M R, Young V M, Faber J E
Appl Microbiol. 1969 Aug;18(2):262-7. doi: 10.1128/am.18.2.262-267.1969.
Blood group activity was proven to be a property of the bacterium per se which possesses it, although such activity was influenced by the cultural environment of the organism. High concentrations of peptones having blood group activity were able to transfer this activity to inactive organisms; however, the conferred activity was proportional to the concentration. As a result of the low concentrations, the blood group activity of peptones was eliminated upon incorporation in culture media, and the activity of the peptones had no effect on the blood group activity levels of microorganisms when grown in these media. Conversely, the vitamin content of culture media did affect blood group active organisms. Multiple vitamins in media decreased the activity levels by blocking the reactive sites of the active organisms on which activity detection depended. Blood group activity levels were highest in media of minimal or no vitamin content. Therefore, it can be concluded that a choice of cultural medium becomes an important factor in the quantitation of bacterial blood group activity.
血型活性被证明是具有该活性的细菌本身的一种特性，尽管这种活性受生物体培养环境的影响。具有血型活性的高浓度蛋白胨能够将这种活性传递给无活性的生物体；然而，赋予的活性与浓度成正比。由于浓度较低，蛋白胨的血型活性在加入培养基后会消失，并且当在这些培养基中生长时，蛋白胨的活性对微生物的血型活性水平没有影响。相反，培养基的维生素含量确实会影响具有血型活性的生物体。培养基中的多种维生素通过阻断活性生物体上活性检测所依赖的反应位点来降低活性水平。在维生素含量极少或没有维生素的培养基中，血型活性水平最高。因此，可以得出结论，培养基的选择成为定量细菌血型活性的一个重要因素。
