de Maeyer-Guignard J, de Maeyer E, Jullien P
Proc Natl Acad Sci U S A. 1969 Jul;63(3):732-9. doi: 10.1073/pnas.63.3.732.
C(3)H/He mice were exposed to total-body X-irradiation of 1000 roentgens and received thereafter 10(7) xenogeneic Wistar rat marrow cells intravenously. In these rat-to-mouse chimeras, serum interferon-producing capacity upon injection of Newcastle disease virus was examined four weeks after grafting. Normal levels of circulating interferon were produced. The interferon, however, had the species specificity of rat interferon, being 20 times more active in rat embryo fibroblasts than in mouse embryo fibroblasts. Moreover, it behaved like rat interferon when filtered on Sephadex G-100 dextran gel, displaying one peak of activity in the 90,000 molecular-weight range and two incompletely separated peaks of 32,000 and 24,000, respectively, in the 30,000 molecular-weight region. The fact that the interferon is of the donor type in rat-to-mouse chimeras permits us to conclude that circulating interferon induced by New-castle disease virus is made in bone-marrow-derived cells.
将C(3)H/He小鼠全身暴露于1000伦琴的X射线照射下，然后静脉注射10(7)个异基因Wistar大鼠骨髓细胞。在这些大鼠-小鼠嵌合体中，移植后四周检查注射新城疫病毒后血清产生干扰素的能力。产生了正常水平的循环干扰素。然而，这种干扰素具有大鼠干扰素的种属特异性，在大鼠胚胎成纤维细胞中的活性比在小鼠胚胎成纤维细胞中高20倍。此外，当在葡聚糖凝胶Sephadex G - 100上过滤时，它表现得像大鼠干扰素，在90,000分子量范围内显示一个活性峰，在30,000分子量区域分别显示两个未完全分离的32,000和24,000的峰。在大鼠-小鼠嵌合体中干扰素是供体类型这一事实使我们能够得出结论，新城疫病毒诱导的循环干扰素是由骨髓来源的细胞产生的。
