Institut du Radium, Université de Paris-Sud, Campus d'Orsay 91405, France.
Infect Immun. 1974 Nov;10(5):1023-8. doi: 10.1128/iai.10.5.1023-1028.1974.
The radioresistant cell systems producing serum interferon after intravenous administration of polyinosinic-polycytidylic acid [poly(I.C)] or encephalomyocarditis virus in mice were studied in rat-to-mouse radiation chimeras. Interferon induced by poly(I.C) became of donor type within 3 months after grafting of irradiated C3H/He mice with Wistar rat bone marrow cells; this indicated that it was made in cells derived from the hemopoietic system. In contrast, encephalomyocarditis virus-induced interferon remained of recipient type in xenogeneic chimeras up to 3 months after grafting, which indicated that the bulk of this interferon originated from a cell population not derived from the hemopoietic system. To ascertain that the respective radiosensitivities of the systems producing rat interferon in chimeras corresponded to that of normal mice, some rat-to-mouse chimeras were subjected to a second X irradiation 1 month after the first irradiation and restoration. Circulating interferon production was studied 4 days later. As expected, the re-irradiation strongly depressed rat serum interferon production induced by Newcastle disease virus but had no effect on rat interferon synthesis induced by poly (I.C). These results point to a macrophage origin for the bulk of poly(I.C)-induced circulating interferon.
在大鼠-小鼠辐射嵌合体中，研究了静脉注射聚肌胞苷酸[poly（I.C）]或脑心肌炎病毒后产生血清干扰素的耐辐射细胞系统。用 Wistar 大鼠骨髓细胞移植照射的 C3H/He 小鼠 3 个月后，poly（I.C）诱导的干扰素成为供体类型；这表明它是由造血系统来源的细胞产生的。相比之下，在异种嵌合体中，脑心肌炎病毒诱导的干扰素在移植后 3 个月内仍保持受者类型，这表明这种干扰素的大部分来自非造血系统来源的细胞群体。为了确定在嵌合体中产生大鼠干扰素的系统的各自放射敏感性与正常小鼠相对应，一些大鼠-小鼠嵌合体在第一次照射和恢复后 1 个月接受第二次 X 照射。4 天后研究了循环干扰素的产生。正如预期的那样，再照射强烈抑制了新城疫病毒诱导的大鼠血清干扰素的产生，但对 poly（I.C）诱导的大鼠干扰素合成没有影响。这些结果表明，聚肌胞苷酸诱导的循环干扰素大部分来源于巨噬细胞。
