Pugh C R, Ultsch G R, Lindahl R
J Exp Zool. 1979 Jul;209(1):65-72. doi: 10.1002/jez.1402090108.
Goniobasis cahawbensis is a stream snail that experiences an annual temperature cycle. G. cochliaris is limited in distribution to springs, and their immediate vicinities, which are characterized by nearly constant annual temperatures. The present study sought to determine whether temperature dependent biochemical differences exist that might account for the differential distribution of these congeneric pleurocerid snails. Eight enzymes were examined following acclimation to 10 degrees, 17 degrees and 24 degrees C. No significant temperature dependent qualitative differences in enzyme phenotypes were demonstrable in either species by starch-gel electrophoresis for malate dehydrogenase, glucose-6-phosphate dehydrogenase, phosphogluconate dehydrogenase, phosphoglucomutase, superoxide dismutase and acetyl and butyryl esterases. Significant quantitative differences were observed in three of these enzymes. G. cahawbensis cytosol malate dehydrogenase activity increased significantly with increasing acclimation temperature, while G. cochliaris malate dehydrogenase activity remained unchanged. The activities of glucose-6-phosphate dehydrogenase did not differ significantly between acclimation temperatures for either species; however, the overall activity of both enzymes was significantly higher for G. cochliaris. Appreciable levels of LDH activity were not demonstrable by electrophoresis or enzymatic assay.
卡哈温斯角螺是一种经历年度温度循环的溪流蜗牛。耳蜗角螺的分布仅限于泉水及其紧邻区域，这些地方的特点是年温度几乎恒定。本研究旨在确定是否存在依赖温度的生化差异，这些差异可能解释这些同属侧鳃螺科蜗牛的不同分布。在适应10摄氏度、17摄氏度和24摄氏度后，检测了八种酶。通过淀粉凝胶电泳对苹果酸脱氢酶、葡萄糖-6-磷酸脱氢酶、磷酸葡萄糖酸脱氢酶、磷酸葡萄糖变位酶、超氧化物歧化酶以及乙酰酯酶和丁酰酯酶进行检测，在这两个物种中均未发现酶表型存在明显的依赖温度的定性差异。在其中三种酶中观察到了显著的定量差异。卡哈温斯角螺胞质苹果酸脱氢酶活性随适应温度升高而显著增加，而耳蜗角螺的苹果酸脱氢酶活性保持不变。两种物种在不同适应温度下葡萄糖-6-磷酸脱氢酶的活性没有显著差异；然而，耳蜗角螺这两种酶的总体活性明显更高。通过电泳或酶促测定均未检测到明显水平的乳酸脱氢酶活性。
