70S核糖体的解离：嗜热脂肪芽孢杆菌和大肠杆菌解离因子的一些特性
Dissociation of 70S ribosomes: some properties of the dissociating factor from Bacillus stearothermophilus and Escherichia coli.
作者信息
Bade E G, González N S, Algranati I S
出版信息
Proc Natl Acad Sci U S A. 1969 Oct;64(2):654-60. doi: 10.1073/pnas.64.2.654.
Abstract
A protein factor which produces in vitro dissociation of 70S particles into 30S and 50S subunits has been obtained from Bacillus stearothermophilus and Escherichia coli. The factor could be extracted from ribosomes, polyribosomes, and S100 supernatant. The kinetics and temperature curve of the dissociation process in the B. stearothermophilus system have been studied and compared with the reaction in the E. coli system. No species specificity was observed when hybrid mixtures of ribosomes and dissociating factor from both bacteria were used. The wide variations of dissociating activity in cells at different stages of growth and the capacity of the liberated subunits to carry out polypeptide synthesis suggest that the dissociating factor has a physiological role.
摘要
一种能使70S颗粒在体外解离成30S和50S亚基的蛋白质因子已从嗜热脂肪芽孢杆菌和大肠杆菌中获得。该因子可从核糖体、多核糖体和S100上清液中提取。对嗜热脂肪芽孢杆菌系统中解离过程的动力学和温度曲线进行了研究，并与大肠杆菌系统中的反应进行了比较。当使用来自两种细菌的核糖体和解离因子的混合混合物时，未观察到物种特异性。在不同生长阶段的细胞中解离活性的广泛变化以及释放的亚基进行多肽合成的能力表明解离因子具有生理作用。
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