70S核糖体在细菌中的生理作用。
Physiological role of 70S ribosomes in bacteria.
作者信息
Algranati I D, Gonzalez N S, Bade E G
出版信息
Proc Natl Acad Sci U S A. 1969 Feb;62(2):574-80. doi: 10.1073/pnas.62.2.574.
Abstract
Evidence is presented indicating that free 70S ribosomes are real components of Bacillus stearothermophilus and Escherichia coli in exponential and stationary phases of growth. After pulses of radioactive leucine or uracil were given, protein synthesis was instantaneously stopped by chloramphenicol, or polypeptide growth was allowed to proceed to completion in slowly cooled cells. Analysis of label distribution in sucrose gradients showed that 70S monomers, and not subunits, are final products of translation.A ribosome cycle for protein synthesis in bacteria is proposed.
摘要
有证据表明，在嗜热脂肪芽孢杆菌和大肠杆菌生长的指数期和稳定期，游离70S核糖体是其真实的组成部分。在给予放射性亮氨酸或尿嘧啶脉冲后，氯霉素可使蛋白质合成瞬间停止，或者在缓慢冷却的细胞中让多肽生长进行至完成。对蔗糖梯度中标记分布的分析表明，翻译的最终产物是70S单体，而非亚基。本文提出了细菌蛋白质合成的核糖体循环。
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