Lawson S N
J Neurocytol. 1979 Jun;8(3):275-94. doi: 10.1007/BF01236123.
A method is described for the analysis of cell types in mouse dorsal root ganglia using the distribution of cell cross-sectional areas measured at the level of the nucleolus in 1.5 micron Epon sections. Using a computer program it was possible to demonstrate the existence of two normally distributed sub-populations of neurons in all the 3rd lumbar segment ganglia (17 in number) measured at various ages from birth to 70 days. The two populations appeared to correspond with large light cells and small dark cells. The large light cell bodies increased in size until about 20 days postnatal, subsequently their size decreased whereas the mean size of the small dark cells reached a plateau by about day 10. The relationship of both nuclear volume and surface area to the surface area of the perikaryon differed between light and dark cells. The number of neurons in L3 remained virtually constant at about 6000 throughout the period examined. Since the proportion of neurons in each population was not shown to change with age there was no evidence that cells could change from one type into the other.
本文描述了一种分析小鼠背根神经节细胞类型的方法，该方法利用在1.5微米厚的环氧树脂切片中，于核仁水平测量的细胞横截面积分布来进行分析。通过一个计算机程序，可以证明在从出生到70天的不同年龄段测量的所有第3腰段神经节（共17个）中，存在两个呈正态分布的神经元亚群。这两个群体似乎分别对应大的亮细胞和小的暗细胞。大的亮细胞体大小在出生后约20天前不断增加，随后其大小减小，而小的暗细胞的平均大小在约第10天时达到稳定水平。亮细胞和暗细胞的核体积和表面积与胞体表面积之间的关系有所不同。在所研究的整个时期内，L3节段的神经元数量实际上保持在约6000个不变。由于没有显示出每个群体中神经元的比例随年龄变化，因此没有证据表明细胞可以从一种类型转变为另一种类型。
