Stuart J, Bitensky L, Chayen J
J Clin Pathol. 1969 Sep;22(5):563-6. doi: 10.1136/jcp.22.5.563.
A new technique for the quantitative estimation of glycolytic and respiratory enzyme activity in intact and unfixed bone marrow and peripheral blood cells is described. The method gives a two- to three-fold increase in demonstrable enzyme activity per cell compared with existing techniques using fixed marrow film preparations, and is particularly applicable to the study of relatively fragile cells such as the leukaemic lymphoblast.
本文描述了一种用于定量估算完整且未固定的骨髓及外周血细胞中糖酵解酶和呼吸酶活性的新技术。与使用固定骨髓涂片制备物的现有技术相比，该方法可使每个细胞的可显示酶活性提高两到三倍，并且特别适用于研究白血病淋巴母细胞等相对脆弱的细胞。
