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未固定白细胞的脱氢酶细胞化学

Dehydrogenase enzyme cytochemistry of unfixed leucocytes.

作者信息

Stuart J, Simpson J S

出版信息

J Clin Pathol. 1970 Sep;23(6):517-21. doi: 10.1136/jcp.23.6.517.

DOI:10.1136/jcp.23.6.517
PMID:5476878
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC476821/
Abstract

An inert, synthetic, sucrose polymer (Ficoll) has been found to have a protective effect on leucocytes during incubation for cytochemical reactions at 37 degrees C. This permits enzyme cytochemistry to be performed on unfixed bone marrow and peripheral blood smears, and on leucocytes cultured in vitro, thus avoiding enzyme inhibition by the use of a fixative. The technique is suitable for the demonstration of both cytoplasmic and intramitochondrial dehydrogenase enzyme activity.

摘要

一种惰性合成蔗糖聚合物（聚蔗糖）已被发现，在37℃进行细胞化学反应孵育期间，对白细胞具有保护作用。这使得酶细胞化学能够在未固定的骨髓和外周血涂片以及体外培养的白细胞上进行，从而避免了因使用固定剂而导致的酶抑制。该技术适用于细胞质和线粒体内脱氢酶活性的显示。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a633/476821/57636d7809a3/jclinpath00086-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a633/476821/6a1fa68a52e4/jclinpath00086-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a633/476821/57636d7809a3/jclinpath00086-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a633/476821/6a1fa68a52e4/jclinpath00086-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a633/476821/57636d7809a3/jclinpath00086-0063-a.jpg

相似文献

1
Dehydrogenase enzyme cytochemistry of unfixed leucocytes.未固定白细胞的脱氢酶细胞化学
J Clin Pathol. 1970 Sep;23(6):517-21. doi: 10.1136/jcp.23.6.517.
2
Enzyme cytochemistry of the energy metabolism in the cells of normal human bone marrow.
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3
[Histochemical differentiation of the cytoplasmic and mitochondrial enzymatic activities in bone marrow cells].[骨髓细胞中细胞质和线粒体酶活性的组织化学分化]
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4
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Quantitative enzyme cytochemistry of leukaemic cells.白血病细胞的定量酶细胞化学
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[Apropos of the method of cytochemical demonstration of some dehydrogenases in human blood cells].[关于人体血细胞中某些脱氢酶的细胞化学显示方法]
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Glycolytic and respiratory enzyme activity in relation to leucocyte maturation.糖酵解和呼吸酶活性与白细胞成熟的关系。
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引用本文的文献

1
Cytochemistry in the bioassay of hormones.激素生物测定中的细胞化学
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2
Quantitative cytochemistry of beta-galactosidase in normal and enzyme deficient (gal) pollen of Brassica campestris: application of the indigogenic method.甘蓝型油菜正常和酶缺陷（gal）花粉中β-半乳糖苷酶的定量细胞化学：靛蓝生成法的应用
Histochem J. 1984 Dec;16(12):1273-96. doi: 10.1007/BF01003726.
3
Routine application of the nitroblue tetrazolium test in the clinical laboratory.硝基四氮唑蓝试验在临床实验室的常规应用。

本文引用的文献

1
Acetone fixation for the cytochemical demonstration of dehydrogenases in blood and bone marrow cells.用于血液和骨髓细胞中脱氢酶细胞化学显示的丙酮固定法。
Nature. 1960 Jul 2;187:85-6. doi: 10.1038/187085a0.
2
Enzyme studies on white blood cells and blood platelets. V. Dehydrogenase activity.白细胞和血小板的酶研究。V. 脱氢酶活性。
Arch Biochem Biophys. 1956 Apr;61(2):278-90. doi: 10.1016/0003-9861(56)90350-2.
3
A cytochemical study of marrow enzymes in megaloblastic anaemia.巨幼细胞贫血骨髓酶的细胞化学研究。
J Clin Pathol. 1973 Jan;26(1):52-6. doi: 10.1136/jcp.26.1.52.
4
Lysosomal enzyme cytochemistry in acute leukaemia.急性白血病中的溶酶体酶细胞化学
J Clin Pathol. 1971 Dec;24(9):831-6. doi: 10.1136/jcp.24.9.831.
5
Polyvinyl alcohol and other tissue protectants in enzyme histochemistry: a consumer's guide.酶组织化学中的聚乙烯醇及其他组织保护剂：消费者指南
Histochem J. 1989 Jul;21(7):373-9. doi: 10.1007/BF01789734.
6
Enzyme cytochemistry of blood and marrow cells.血液和骨髓细胞的酶细胞化学
Histochem J. 1975 Sep;7(5):471-87. doi: 10.1007/BF01003884.
7
beta-Hydroxybutyrate dehydrogenase activity in human and kitten odontoclasts and kitten osteoclasts.人类和小猫破牙细胞以及小猫破骨细胞中的β-羟基丁酸脱氢酶活性。
Histochem J. 1978 Nov;10(6):731-7. doi: 10.1007/BF01003122.
8
Enzyme histochemical properties of kitten osteoclasts in bone imprint preparations.幼猫破骨细胞在骨压片标本中的酶组织化学特性
Histochem J. 1978 Nov;10(6):645-56. doi: 10.1007/BF01003116.
9
Enzyme histochemical characteristics of human and kitten odontoclasts and kitten osteoclasts: a comparative study using whole cells.人和幼猫破牙细胞及幼猫破骨细胞的酶组织化学特征：一项使用全细胞的比较研究
Histochem J. 1979 Nov;11(6):719-35. doi: 10.1007/BF01004735.
Br J Haematol. 1968 Nov;15(5):443-54. doi: 10.1111/j.1365-2141.1968.tb01564.x.
4
Quantitative enzyme cytochemistry of leukaemic cells.白血病细胞的定量酶细胞化学
J Clin Pathol. 1969 Sep;22(5):563-6. doi: 10.1136/jcp.22.5.563.
5
Cytochemical demonstration of beta-glucuronidase activity in blood and bone marrow cells.血液和骨髓细胞中β-葡萄糖醛酸酶活性的细胞化学证明
J Histochem Cytochem. 1967 Aug;15(11):680-7. doi: 10.1177/15.11.680.