Chang Y T, Andersen R N
J Bacteriol. 1969 Sep;99(3):867-75. doi: 10.1128/jb.99.3.867-875.1969.
Studies were made on morphological changes of Mycobacterium lepraemurium grown in cultures of mouse peritoneal macrophages. Two types of nonsolid or irregularly stained M. lepraemurium were observed. One type occurred in the growth phase of the organisms during the stage of preparation for bacillary multiplication. The nonsolid bacilli appeared as elongated organisms having pointed ends, isolated acid-fast dots, or faintly stained areas at the ends of the bacilli. It is possible that this irregularity in staining is due to a very gradual, versus an instantaneous, acquisition of acid-fast material during bacillary multiplication and maturation. Solid forms were again observed upon maturation. Nonsolid bacilli were also observed in macrophage cultures infected with autoclave-killed M. lepraemurium. Under these conditions there was an emergence of organisms which showed irregularly stained areas and various forms of deformity unaccompanied by elongation or multiplication. These irregularities were most probably due to the destructive process of digestion of bacillary protoplasm. The present study does not support the current hypothesis that all nonsolid acid-fast organisms are nonviable.
对在小鼠腹腔巨噬细胞培养物中生长的鼠麻风分枝杆菌的形态变化进行了研究。观察到两种类型的非固态或染色不规则的鼠麻风分枝杆菌。一种类型出现在细菌繁殖准备阶段生物体的生长阶段。非固态杆菌表现为两端尖锐的细长生物体、孤立的抗酸小点或杆菌末端的淡染区域。这种染色不规则可能是由于在细菌繁殖和成熟过程中，抗酸物质的获取是非常渐进的，而不是瞬间的。成熟时再次观察到固态形式。在感染了经高压灭菌杀死的鼠麻风分枝杆菌的巨噬细胞培养物中也观察到了非固态杆菌。在这些条件下，出现了一些生物体，它们显示出染色不规则的区域和各种形式的畸形，没有伸长或繁殖。这些不规则很可能是由于细菌原生质消化的破坏过程。本研究不支持当前所有非固态抗酸生物体都无活力的假说。
