白细胞介素-10对巨噬细胞的治疗可增强麻风分枝杆菌在细胞内的存活能力。

IL-10 treatment of macrophages bolsters intracellular survival of Mycobacterium leprae.

作者信息

Fukutomi Yasuo, Matsuoka Masanori, Minagawa Fumishige, Toratani Satoshi, McCormick Gregory, Krahenbuhl James

机构信息

Leprosy Research Center, National institute of Infectious Diseases, Tokyo, Japan.

出版信息

Int J Lepr Other Mycobact Dis. 2004 Mar;72(1):16-26. doi: 10.1489/1544-581X(2004)072<0016:ITOMBI>2.0.CO;2.

DOI:10.1489/1544-581X(2004)072<0016:ITOMBI>2.0.CO;2

PMID:15217319

Abstract

In these studies, metabolically active Mycobacterium leprae were maintained for as long as 8 weeks in monolayer cultures of mouse peritoneal macrophages (MPhi). Supplemental IL-10, but not TGF-beta, bolstered, directly or indirectly, M. leprae metabolism in mouse MPhi. In the cell culture system temperature setting is extremely important and 31 to 33 degrees C incubation temperature was more permissive than 37 degrees C. Acid fast staining and transmission electron microscopy (TEM) of intracellular M. leprae revealed visible elongation of bacilli cultured under the above ideal conditions.

摘要

在这些研究中，代谢活跃的麻风分枝杆菌在小鼠腹腔巨噬细胞（MPhi）的单层培养物中维持长达8周。补充白细胞介素-10而非转化生长因子-β，直接或间接地增强了小鼠MPhi中麻风分枝杆菌的代谢。在细胞培养系统中，温度设置极为重要，31至33摄氏度的孵育温度比37摄氏度更适宜。对细胞内麻风分枝杆菌进行抗酸染色和透射电子显微镜（TEM）观察发现，在上述理想条件下培养的杆菌可见伸长。