大肠菌产生L-天冬酰胺酶2的条件。
Conditions for the production of L-asparaginase 2 by coliform bacteria.
作者信息
Bilimoria M H
出版信息
Appl Microbiol. 1969 Dec;18(6):1025-30. doi: 10.1128/am.18.6.1025-1030.1969.
Abstract
Of 28 coliforms, five strains of Escherichia coli were particularly active in elaborating L-asparaginase 2, the form of the enzyme useful in the treatment of some forms of cancer. Since it is advantageous to start purification of the enzyme from highly active cells, cultural conditions necessary for good growth and high enzyme yield have been studied. Gentle aeration proved suitable for good growth as well as high enzyme content. Stationary cultures gave poor growth, whereas vigorous aeration gave good growth but resulted in a marked decrease in the enzyme content of the cells. L-Asparaginase 2 has been purified about 40-fold by a combination of ammonium sulfate and ethyl alcohol precipitations.
摘要
在28株大肠菌群中，有5株大肠杆菌在产生L-天冬酰胺酶2方面表现得尤为活跃，这种酶的形式可用于治疗某些癌症。由于从高活性细胞开始纯化这种酶具有优势，因此已经研究了良好生长和高酶产量所需的培养条件。事实证明，温和通气适合良好生长以及高酶含量。静置培养生长不佳，而剧烈通气生长良好，但导致细胞中的酶含量显著下降。通过硫酸铵沉淀和乙醇沉淀相结合的方法，L-天冬酰胺酶2已被纯化了约40倍。
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本文引用的文献
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Evidence that the L-asparaginase of guinea pig serum is responsible for its antilymphoma effects. II. Lymphoma 6C3HED cells cultured in a medium devoid of L-asparagine lose their susceptibility to the effects of guinea pig serum in vivo.豚鼠血清中的L-天冬酰胺酶是其抗淋巴瘤作用的原因的证据。II. 在不含L-天冬酰胺的培养基中培养的淋巴瘤6C3HED细胞对豚鼠血清在体内的作用失去敏感性。
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