用于区分克雷伯菌属和肠杆菌属分离株的十分钟检测法。
Ten-minute test for differentiating between Klebsiella and Enterobacter isolates.
作者信息
Matsen J M
出版信息
Appl Microbiol. 1970 Mar;19(3):438-40. doi: 10.1128/am.19.3.438-440.1970.
Abstract
A 10-minute test, utilizing a urease paper-reagent strip (PATHO-TEC), for differentiating Klebsiella and Enterobacter species is described. By using a heavy suspension of organisms and 50 C temperature for incubation, 93% of Klebsiella strains (186/200) were positive and 95% of Enterobacter strains (190/200) were negative with this testing system. The rapid nature of the test (10 min), the facility with which it can be carried out, and the ease with which the strips can be stored and handled may make this a useful aid for the clinical microbiologist.
摘要
本文描述了一种利用尿素酶试纸条（PATHO-TEC）区分克雷伯菌属和肠杆菌属菌种的10分钟检测方法。通过使用菌体重悬液并在50℃温度下孵育，该检测系统显示93%的克雷伯菌菌株（186/200）呈阳性，95%的肠杆菌菌株（190/200）呈阴性。该检测方法快速（10分钟）、操作简便、试纸条易于储存和处理，可能会成为临床微生物学家的有用辅助手段。
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