Barry A L, Bernsohn K L, Thrupp L D
Appl Microbiol. 1969 Aug;18(2):156-8. doi: 10.1128/am.18.2.156-158.1969.
Infections with Klebsiella and Enterobacter have increased among hospitalized patients. To study such infections, relatively simple but precise methods are needed for clinical laboratories to identify the two genera accurately. Moreover, a rapid identification is essential for assisting with the therapy of the patients. For this purpose, a new 4-hr urease test was developed so that colonies could be tested directly from blood-agar plates which have been inoculated with clinical material and allowed to incubate overnight. This 4-hr test was positive with 98.5% of 202 Klebsiella species and negative with 80 Enterobacter species. As a single criterion for distinguishing between the two major genera, the new 4-hr urease test was just as accurate as a motility test (99% of the 282 isolates were accurately identified with either). The 4-hr urease test represents a simple, rapid, and reliable technique which is ideally suited for use in clinical microbiology laboratories.
住院患者中肺炎克雷伯菌和阴沟肠杆菌感染有所增加。为研究此类感染，临床实验室需要相对简单但精确的方法来准确鉴定这两个菌属。此外，快速鉴定对于协助患者治疗至关重要。为此，开发了一种新的4小时尿素酶试验，以便可以直接从接种了临床样本并过夜培养的血琼脂平板上对菌落进行检测。在202株肺炎克雷伯菌中，该4小时试验的阳性率为98.5%，而80株阴沟肠杆菌的试验结果均为阴性。作为区分这两个主要菌属的单一标准，新的4小时尿素酶试验与动力试验一样准确（282株分离株中99%通过二者之一被准确鉴定）。4小时尿素酶试验是一种简单、快速且可靠的技术，非常适合临床微生物实验室使用。
