Brown C A
Br J Exp Pathol. 1970 Apr;51(2):203-9.
An established line of rat fibroblasts (14pf), growing in either of two media, was infected with One of the media (equal parts of human cord serum and Hanks' balanced salt solution) supports active intracellular bacterial multiplication, whereas the other (10 per cent calf serum in Eagle's medium with added peptone) fails to do so. Within 1 week of infection, the cells were stained for acid phosphatase, aryl sulphatase and β-galactosidase activities and examined by light microscopy. Cells in equal parts of cord serum and Hanks' balanced salt solution showed an accumulation of all 3 enzymes around the bacteria whereas cells in 10 per cent calf serum in Eagle's medium showed only light, diffuse, general staining with little localization around the bacteria. Cells in the former medium also had greater lysosomal activity before infection with than cells in the latter medium, showing that the increased activity was not the result of intracellular multiplication of the bacteria. It is concluded that the failure to support mycobacterial growth by the cells growing in the medium containing calf serum must be unconnected with direct lysosomal action. A possible beneficial effect of lysosomal enzymes on the growth of these obligate intracellular mycobacteria is suggested.
一种已建立的大鼠成纤维细胞系（14pf），在两种培养基中的任何一种中生长，被感染。其中一种培养基（人脐带血清和汉克斯平衡盐溶液等份混合）支持细胞内细菌的活跃增殖，而另一种（含有添加蛋白胨的伊格尔培养基中的10%小牛血清）则不能。在感染后1周内，对细胞进行酸性磷酸酶、芳基硫酸酯酶和β-半乳糖苷酶活性染色，并通过光学显微镜检查。在脐带血清和汉克斯平衡盐溶液等份混合的培养基中的细胞，在细菌周围显示出所有三种酶的积累，而在含有10%小牛血清的伊格尔培养基中的细胞仅显示出轻微的、弥漫性的、总体染色，在细菌周围几乎没有定位。在前一种培养基中的细胞在感染前也比后一种培养基中的细胞具有更高的溶酶体活性，表明活性增加不是细菌细胞内增殖的结果。得出的结论是，在含有小牛血清的培养基中生长的细胞不能支持分枝杆菌生长，这一定与溶酶体的直接作用无关。提示溶酶体酶对这些专性细胞内分枝杆菌的生长可能具有有益作用。
