大肠杆菌甲酰甲硫氨酰转运核糖核酸的结构与功能。I.嘧啶残基修饰对氨酰合成酶识别的影响。
Structure and function of E. coli formylmethionyl tRNA. I. Effect of modification of pyrimidine residues on aminoacyl synthetase recognition.
作者信息
Schulman L H
出版信息
Proc Natl Acad Sci U S A. 1970 Jun;66(2):507-14. doi: 10.1073/pnas.66.2.507.
Abstract
E. coli formylmethionyl tRNA (tRNA(fMet)) has been irradiated with ultraviolet light in the presence of Mg(2+) to the extent of 50 per cent inactivation of amino acid acceptance. Separation of active and inactive molecules after irradiation has shown that ultraviolet light modification of the uridine in the anticodon, the uridine in the small loop, the 4-thiouridine, and the pyrimidines in the double-stranded stem adjacent to the dihydrouridine loop has no effect on aminoacylation or transformylation. The ultraviolet light-induced inactivation of methionine acceptance by tRNA(fMet) is due almost entirely to modification of the cytidine residues in the 3'-terminal CCA-OH sequence.
摘要
在Mg(2+)存在的情况下，用紫外线照射大肠杆菌甲酰甲硫氨酰tRNA（tRNA(fMet)），使其氨基酸接受活性失活50%。照射后对活性和非活性分子进行分离，结果表明，反密码子中的尿苷、小环中的尿苷、4-硫尿苷以及与二氢尿苷环相邻的双链茎中的嘧啶经紫外线修饰后，对氨酰化或甲酰化没有影响。tRNA(fMet)因紫外线诱导而导致的甲硫氨酸接受活性失活几乎完全是由于3'-末端CCA-OH序列中的胞苷残基发生了修饰。
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