高甲基化大肠杆菌甲硫氨酸起始转运核糖核酸的受体活性
Acceptor activity of hypermethylated E. coli tRNAf-Met.
作者信息
Shershneva L P, Venkstern T V, Bayev A A
出版信息
Nucleic Acids Res. 1974 Feb;1(2):235-43. doi: 10.1093/nar/1.2.235.
Abstract
The acceptor activity of normal E. coli tRNA(Met) (f) was compared with that of a preparation with surplus methyl groups introduced by a crude methylase preparation from rat hepatoma. No changes in charging were detected when the aminoacylation was carried out in a homologous system. The data indicate that neither the surplus methyl groups by themselves, nor the eventual changes in spatial arrangement are essential for charging of E. coli tRNA(Met) (f).
摘要
将正常大肠杆菌tRNA(Met)(f)的接受体活性与用来自大鼠肝癌的粗甲基化酶制剂引入多余甲基的制剂的接受体活性进行了比较。当在同源系统中进行氨酰化时，未检测到电荷变化。数据表明，多余的甲基本身以及空间排列的最终变化对于大肠杆菌tRNA(Met)(f)的电荷化都不是必需的。
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