Norman M C, Franck E B, Choate R V
Appl Microbiol. 1970 Jul;20(1):69-71. doi: 10.1128/am.20.1.69-71.1970.
Procedures for maintaining Mycoplasma strains are described. Liquid nitrogen storage provided an adequate means for keeping stock cultures stable. Over 74 strains of approximately 26 species have been preserved in this way, some for as long as 9 years. Mycoplasma strains can also be recovered satisfactorily from the freeze-dried state when the procedure described includes the use of 12% (v/v) sucrose as an additive. Fifteen strains representing 12 or more species were subjected to a freeze-drying program with and without sucrose added to concentrated cell suspensions in growth medium. Cell counts indicate improved survival with sucrose.
描述了支原体菌株的保存方法。液氮保存为保持储备培养物稳定提供了一种合适的手段。已通过这种方式保存了约26个物种的74株以上菌株，有些已保存长达9年。当所述程序包括使用12%（v/v）蔗糖作为添加剂时，也可以从冻干状态令人满意地复苏支原体菌株。将代表12个或更多物种的15株菌株在生长培养基中的浓缩细胞悬液中进行添加或不添加蔗糖的冻干程序。细胞计数表明添加蔗糖可提高存活率。
