多核苷酸的酶促连接。IX. 通过测量线性脱氧腺苷酸-脱氧胸苷酸共聚物形成环来简单快速地测定多核苷酸连接（连接酶）活性。 - Suppr

Suppr

超能文献

Enzymatic joining of polynucleotides. IX. A simple and rapid assay of polynucleotide joining (ligase) activity by measurement of circle formation from linear deoxyadenylate-deoxythymidylate copolymer.

作者信息

Modrich P, Lehman I R

出版信息

J Biol Chem. 1970 Jul 25;245(14):3626-31.

PMID:4919213

Abstract

摘要

相似文献

Enzymatic joining of polynucleotides. IX. A simple and rapid assay of polynucleotide joining (ligase) activity by measurement of circle formation from linear deoxyadenylate-deoxythymidylate copolymer.多核苷酸的酶促连接。IX. 通过测量线性脱氧腺苷酸-脱氧胸苷酸共聚物形成环来简单快速地测定多核苷酸连接（连接酶）活性。

J Biol Chem. 1970 Jul 25;245(14):3626-31.

Enzymic joining of polynucleotides. IV. Formation of a circular deoxyadenylate-deoxythymidylate copolymer.多核苷酸的酶促连接。IV. 环状脱氧腺苷酸-脱氧胸苷酸共聚物的形成。

J Mol Biol. 1968 Sep 14;36(2):275-85. doi: 10.1016/0022-2836(68)90381-1.

Polynucleotide ligase-catalyzed joining of deoxyribo-oligonucleotides on ribopolynucleotide templates and of ribo-oligonucleotides on deoxyribopolynucleotide templates.多核苷酸连接酶催化脱氧核糖寡核苷酸在核糖多核苷酸模板上的连接以及核糖寡核苷酸在脱氧核糖多核苷酸模板上的连接。

Proc Natl Acad Sci U S A. 1970 Sep;67(1):68-73. doi: 10.1073/pnas.67.1.68.

Enzymatic breakage and joining of deoxyribonucleic acid. IX. Synthesis and properties of the deoxyribonucleic acid adenylate in the phage T4 ligase reaction.脱氧核糖核酸的酶促断裂与连接。IX. 噬菌体T4连接酶反应中脱氧核糖核酸腺苷酸的合成与性质

J Biol Chem. 1971 Jul 25;246(14):4523-30.

Enzymatic joining of polynucleotides. VI. Activity of a synthetic adenylylated polydeoxynucleotide in the reaction.多核苷酸的酶促连接。VI. 合成的腺苷酸化多脱氧核苷酸在该反应中的活性。

J Biol Chem. 1969 Jan 10;244(1):43-7.

Enzymatic removal and replacement of nucleotides at single strand breaks in deoxyribonucleic acid.脱氧核糖核酸单链断裂处核苷酸的酶促去除与替换

J Biol Chem. 1971 Apr 25;246(8):2680-91.

On the fidelity of phage T4-induced polynucleotide ligase in the joining of chemically synthesized deoxyribooligonucleotides.噬菌体T4诱导的多核苷酸连接酶在化学合成脱氧核糖寡核苷酸连接中的保真度

Biochem Biophys Res Commun. 1970 May 22;39(4):631-6. doi: 10.1016/0006-291x(70)90251-2.

Ezymatic breakage and joining of deoxyribonucleic acid. VII. Properties of the enzyme-adenylate intermediate in the polynucleotide ligase reaction.脱氧核糖核酸的酶促断裂与连接。VII. 多核苷酸连接酶反应中酶 - 腺苷酸中间体的性质。

J Biol Chem. 1968 Sep 10;243(17):4556-63.

Enzymatic joining of polynucleotides, V. A DNA-adenylate intermediate in the polynucleotide-joining reaction.多核苷酸的酶促连接，V. 多核苷酸连接反应中的DNA - 腺苷酸中间体

Proc Natl Acad Sci U S A. 1968 Sep;61(1):237-44. doi: 10.1073/pnas.61.1.237.

Preparation of a circular bihelical deoxyribonucleic acid containing repeating dinucleotide sequences.含有重复二核苷酸序列的环状双螺旋脱氧核糖核酸的制备

Biochemistry. 1971 Apr 27;10(9):1511-21. doi: 10.1021/bi00785a001.

引用本文的文献

Expression of DNA ligase genes by ram spermatid nuclei and RNA in amphibian eggs.DNA连接酶基因在绵羊精子细胞核及两栖类卵子RNA中的表达

Rouxs Arch Dev Biol. 1986 Apr;195(3):186-192. doi: 10.1007/BF02439437.

The NAD-dependent ligase encoded by yerG is an essential gene of Bacillus subtilis.由yerG编码的NAD依赖性连接酶是枯草芽孢杆菌的一个必需基因。

Nucleic Acids Res. 2000 Dec 1;28(23):4642-8. doi: 10.1093/nar/28.23.4642.

Specific inhibition of the eubacterial DNA ligase by arylamino compounds.芳基氨基化合物对真细菌DNA连接酶的特异性抑制作用。

Antimicrob Agents Chemother. 1999 Nov;43(11):2766-72. doi: 10.1128/AAC.43.11.2766.

L-ATP is recognized by some cellular and viral enzymes: does chance drive enzymic enantioselectivity?L-ATP被一些细胞酶和病毒酶所识别：是偶然性驱动酶的对映体选择性吗？

Biochem J. 1999 Feb 1;337 ( Pt 3)(Pt 3):585-90.

Characterization of human DNA ligase I expressed in a baculovirus-insect cell system.在杆状病毒-昆虫细胞系统中表达的人DNA连接酶I的特性分析。

Biochem J. 1995 Dec 1;312 ( Pt 2)(Pt 2):593-7. doi: 10.1042/bj3120593.

Bacteriophage T4 and human type I DNA ligases relax DNA under joining conditions.噬菌体T4和人类I型DNA连接酶在连接条件下使DNA松弛。

Nucleic Acids Res. 1993 Dec 25;21(25):5934-9. doi: 10.1093/nar/21.25.5934.

Autonomously replicating sequences in Saccharomyces cerevisiae.酿酒酵母中的自主复制序列。

Proc Natl Acad Sci U S A. 1980 Nov;77(11):6329-33. doi: 10.1073/pnas.77.11.6329.

Control of DNA ligase molecular forms in nucleocytoplasmic combinations of axolotl and Pleurodeles.蝾螈和真螈核质组合中DNA连接酶分子形式的调控

Proc Natl Acad Sci U S A. 1983 Jun;80(11):3368-71. doi: 10.1073/pnas.80.11.3368.

Enzymatic synthesis, ligation, and restriction of DNA containing deoxy-4-thiothymidine.含脱氧-4-硫代胸苷的DNA的酶促合成、连接和限制

Nucleic Acids Res. 1981 Feb 25;9(4):753-67. doi: 10.1093/nar/9.4.753.

Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase.具有改变的DNA连接酶的大肠杆菌突变体的酶学特性分析。

Proc Natl Acad Sci U S A. 1971 May;68(5):1002-5. doi: 10.1073/pnas.68.5.1002.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验