大肠杆菌中耐药传递因子脱氧核糖核酸的研究。
Studies on resistance transfer factor deoxyribonucleic acid in Escherichia coli.
作者信息
Silver R P, Falkow S
出版信息
J Bacteriol. 1970 Oct;104(1):340-4. doi: 10.1128/jb.104.1.340-344.1970.
Abstract
A variant of the derepressed R factor, R1, which does not contain any of the drug resistance markers, and represents, in large part, the resistance transfer factor (RTF) was studied in Escherichia coli. RTF deoxyribonucleic acid (DNA) was specifically labeled in a female cell after conjugation. Physical characterization of the molecule showed that RTF possessed an average molecular weight of 50 x 10(6) daltons and a buoyant density of 1.709 g/cm(3). By comparison to R1, we calculate that the region of DNA carrying the drug resistance genes is therefore about 20% of the R1 molecule and has a buoyant density of approximately 1.716 g/cm(3). These results support the hypothesis that the single species of R-factor DNA observed in E. coli represents a composite of the 1.709 and 1.716 g/cm(3) replicons seen in Proteus.
摘要
对去阻遏R因子R1的一个变体进行了研究，该变体不含任何耐药标记，在很大程度上代表耐药转移因子（RTF），研究对象为大肠杆菌。在接合后，雌性细胞中的RTF脱氧核糖核酸（DNA）被特异性标记。该分子的物理特性表明，RTF的平均分子量为50×10⁶道尔顿，浮力密度为1.709 g/cm³。通过与R1比较，我们计算得出携带耐药基因的DNA区域约占R1分子的20%，浮力密度约为1.716 g/cm³。这些结果支持了这样一种假说，即在大肠杆菌中观察到的单一R因子DNA代表了在变形杆菌中看到的1.709和1.716 g/cm³复制子的复合体。
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