细菌中的非染色体抗生素抗性。3. R 因子 R1 离散转移单位的分离。
Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1.
作者信息
Cohen S N, Miller C A
出版信息
Proc Natl Acad Sci U S A. 1970 Oct;67(2):510-6. doi: 10.1073/pnas.67.2.510.
Abstract
A covalently-closed circular DNA species, banding at a buoyant density of rho = 1.709 g/cm(3) in CsCl, has been identified in antibiotic-sensitive colonies of E. coli strain AB2463 (rec A(-)) after mating with a Proteus mirabilis strain that carries the R-factor, R1. This plasmid, which represents a stable segregant of R1 that has lost all of the drug resistance determinants present on the parent R-factor but which has retained its ability to be transferred by conjugation, fulfills the functional definition of the R-factor transfer unit (RTF).
摘要
在大肠杆菌AB2463菌株（rec A(-)）的抗生素敏感菌落中，与携带R因子R1的奇异变形杆菌菌株交配后，鉴定出一种共价闭合环状DNA物种，其在CsCl中的浮力密度为ρ = 1.709 g/cm³。该质粒是R1的稳定分离物，它失去了亲本R因子上存在的所有耐药决定簇，但保留了通过接合转移的能力，符合R因子转移单位（RTF）的功能定义。
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