大肠杆菌K-12中胸苷代谢的调控：超声提取物中1,5-磷酸脱氧核糖变位酶测定的最佳条件。
Regulation of thymidine metabolism in Escherichia coli K-12: optimal conditions for the assay of 1,5-phosphodeoxyribomutase in ultrasonic extracts.
作者信息
Bonney R J, Weinfeld H
出版信息
J Bacteriol. 1970 Sep;103(3):650-5. doi: 10.1128/jb.103.3.650-655.1970.
Abstract
Assay conditions have been established which allow maximum expression of phosphodeoxyribomutase activity in ultrasonic extracts of Escherichia coli K-12. The enzyme requires 2-mercaptoethanol, manganous ions, and glucose 1,6-diphosphate for optimal activity. When cells are grown in minimal medium with glycerol as the carbon source and supplemented with 10(-3)m thymidine, phosphodeoxyribomutase is induced three- to four-fold. Cell pellets may be frozen for 24 hr before sonic disruption without loss of enzyme activity. The assay is highly reproducible.
摘要
已经建立了测定条件，可使大肠杆菌K - 12超声提取物中的磷酸脱氧核糖变位酶活性得到最大程度的表达。该酶需要2 - 巯基乙醇、锰离子和葡萄糖1,6 - 二磷酸才能达到最佳活性。当细胞在以甘油作为碳源的基本培养基中生长并补充10(-3)m胸苷时，磷酸脱氧核糖变位酶的诱导倍数为三到四倍。细胞沉淀在超声破碎前可冷冻24小时而不会损失酶活性。该测定具有高度的可重复性。
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