Wynder E L, Hoffmann D
Br J Cancer. 1970 Sep;24(3):574-87. doi: 10.1038/bjc.1970.70.
In tobacco carcinogenesis research, considerable attention has been paid to the choice of the bioassay. The ideal system should simulate the human smoking setting as closely as possible and should utilize tissue of a type similar to that found at the sites where the tobacco smoke-related cancers originate in man. However, although certain inhalation experiments in the laboratory meet these requirements to some extent, they are generally timeconsuming and difficult to evaluate and since they usually have to be performed on large animals, are extremely costly when used for the identification of the actual tumorigenic agents in the smoke.The present article examines the reasons why mouse skin is a useful bioassay. The system has enabled investigators to identify tumour initiators and accelerators and to determine that the major tumour promoters reside in the weakly acidic portion of tobacco smoke. The mouse skin bioassay demonstrated that with significant inhibition of the pyrosynthesis of alkylated and non-alkylated polynuclear aromatic hydrocarbons, the tumorigenicity of the "tar" will also decrease significantly.
在烟草致癌作用研究中，生物测定法的选择受到了相当大的关注。理想的系统应尽可能贴近地模拟人类吸烟环境，并且应使用与人类烟草烟雾相关癌症起源部位所发现的组织类型相似的组织。然而，尽管实验室中的某些吸入实验在一定程度上满足了这些要求，但它们通常耗时且难以评估，而且由于通常必须在大型动物身上进行，用于识别烟雾中实际致癌剂时成本极高。本文探讨了小鼠皮肤为何是一种有用的生物测定法的原因。该系统使研究人员能够识别肿瘤引发剂和促进剂，并确定主要的肿瘤促进剂存在于烟草烟雾的弱酸性部分。小鼠皮肤生物测定法表明，随着烷基化和非烷基化多环芳烃热合成的显著抑制，“焦油”的致瘤性也将显著降低。
