Duchen L W
J Neurol Neurosurg Psychiatry. 1970 Oct;33(5):580-5. doi: 10.1136/jnnp.33.5.580.
A sublethal quantity of botulinum toxin was injected into the muscles of one leg in mice. The histochemical localization of succinate dehydrogenase (SD) and phosphorylase (P) in soleus and gastrocnemius was studied at intervals of from one week to 15 months after the injection of toxin. In normal mice the fibres of soleus are rich in SD and poor in P, but in the superficial part of gastrocnemius the majority of fibres are rich in P and poor in SD. After the injection of toxin the muscles became paralysed and atrophied for several weeks during which time the fibres of gastrocnemius lost their staining reactivity for P and the fibres of soleus showed a weakening of the SD reaction. With the recovery from the effects of the toxin the muscle fibres increased in size and in P activity. Recovery commenced sooner in soleus than in gastrocnemius and for several weeks soleus fibres showed a stronger reaction for P than normal, but with longer survival the enzyme reactions in soleus became normal. Recovery in gastrocnemius began about six weeks after the injection of toxin. Many muscle fibres in gastrocnemius became much enlarged while others remained atrophied. Many fibres gave a strong histochemical reaction for both enzymes. These abnormalities in gastrocnemius seemed to be permanent.
将亚致死剂量的肉毒杆菌毒素注射到小鼠一条腿的肌肉中。在注射毒素后1周~15个月的时间间隔内，研究了比目鱼肌和腓肠肌中琥珀酸脱氢酶（SD）和磷酸化酶（P）的组织化学定位。在正常小鼠中，比目鱼肌纤维富含SD而P含量低，但在腓肠肌浅层，大多数纤维富含P而SD含量低。注射毒素后，肌肉麻痹并萎缩数周，在此期间，腓肠肌纤维对P失去染色反应性，比目鱼肌纤维的SD反应减弱。随着毒素作用的恢复，肌纤维大小和P活性增加。比目鱼肌的恢复比腓肠肌开始得早，并且在数周内比目鱼肌纤维对P的反应比正常情况更强，但随着存活时间延长，比目鱼肌中的酶反应恢复正常。腓肠肌的恢复在注射毒素后约6周开始。腓肠肌中的许多肌纤维变得明显增大，而其他纤维仍萎缩。许多纤维对这两种酶都有强烈的组织化学反应。腓肠肌中的这些异常似乎是永久性的。
