Gaddum P, Blandau R J
Science. 1970 Nov 13;170(3959):749-51. doi: 10.1126/science.170.3959.749.
The acrosomes of spermatozoa of several mammalian species show proteolytic activity when applied to fixed gelatin membranes. The technique permits continuous observation of the enzymatic reaction of an individual spermatozoon. Release of the enzyme occurs solely in the region of the acrosome, in a manner which is species-specific.
几种哺乳动物精子的顶体在应用于固定的明胶膜时显示出蛋白水解活性。该技术允许对单个精子的酶促反应进行连续观察。酶的释放仅发生在顶体区域,其方式具有物种特异性。