Datta B, Poddar R K
J Virol. 1970 Nov;6(5):583-8. doi: 10.1128/JVI.6.5.583-588.1970.
Four types of phiX-infected cells of Escherichia coli CR, a thymine-requiring strain of E. coli C, were prepared in which the parental replicative-form deoxyribonucleic acid (RF DNA) was labeled with same specific amounts of bromouracil in (i) both strands, (ii) only the infecting viral strand, (iii) only the complementary strand, and (iv) neither strand. The sensitivity of each type of infected cell toward irradiation by ultraviolet light, visible light, and X rays was measured. The results indicate that a certain amount of radiation damage in the infecting viral strand of the parental RF was more inhibitory to the production of progeny phage than when the damage was in the complementary strand. Similar conclusions were also drawn from "suicide" experiments of the phage-infected complexes containing (32)P of the same specific activity on either strand of the parental RF DNA. The results suggest that the beta decay occurring in the infecting viral strand was more effective in inactivating the plaque-forming ability of the complex.
制备了大肠杆菌CR（大肠杆菌C的胸腺嘧啶需求菌株）的四种被噬菌体φX感染的细胞类型，其中亲代复制型脱氧核糖核酸（RF DNA）在以下情况中用相同特定量的溴尿嘧啶进行标记：（i）两条链均标记；（ii）仅感染性病毒链标记；（iii）仅互补链标记；（iv）两条链均未标记。测定了每种感染细胞类型对紫外线、可见光和X射线照射的敏感性。结果表明，亲代RF的感染性病毒链中一定量的辐射损伤比损伤位于互补链时对子代噬菌体的产生更具抑制作用。从对亲代RF DNA任一条链含有相同比活度的（32）P的噬菌体感染复合物的“自杀”实验中也得出了类似结论。结果表明，感染性病毒链中发生的β衰变在使复合物的噬菌斑形成能力失活方面更有效。
