光动力学灭活噬菌体φX174单链DNA细胞内复制阻滞的鉴定
Identification of the block in the intracellular replication of single-stranded DNA of photodynamically inactivated bacteriophage phi X174.
作者信息
Chaudhuri U C, Poddar R K
出版信息
J Virol. 1973 Mar;11(3):368-71. doi: 10.1128/JVI.11.3.368-371.1973.
Abstract
(32)P-labeled single-stranded DNA phage phiX174 was photodynamically inactivated by irradiation in air with visible light in the presence of the acridine dye, proflavine sulfate. The inactivated phages could adsorb to the host cells but failed to lyse them. Formation of intracellular mature phages was almost completely inhibited. Photodynamic lesions in phiX174 DNA caused intracellular formation of defective double-stranded replicative form molecules which ultimately reverted to the single-stranded configuration.
摘要
用吖啶黄染料硫酸原黄素处理后，在空气中用可见光照射，可使32P标记的单链DNA噬菌体φX174发生光动力失活。失活的噬菌体能够吸附到宿主细胞上，但无法裂解它们。细胞内成熟噬菌体的形成几乎完全受到抑制。φX174 DNA中的光动力损伤导致细胞内形成有缺陷的双链复制型分子，这些分子最终又恢复为单链构型。
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