在蛋白质 - DNA - RNA 复合物中分离出的大肠杆菌折叠基因组。
The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex.
作者信息
Stonington O G, Pettijohn D E
出版信息
Proc Natl Acad Sci U S A. 1971 Jan;68(1):6-9. doi: 10.1073/pnas.68.1.6.
Abstract
The DNA of Escherichia coli has been isolated in a compact structure containing small amounts of protein and RNA and having a sedimentation coefficient of approximately 3200 S. The molecular weight of the DNA in the complex is very large (probably higher than 10(9)); the protein is predominantly core RNA polymerase; the RNA is chiefly nascent messenger and ribosomal chains. Solutions containing the complex have low viscosities; this plus its sedimentation rate suggest that the DNA is in a tightly folded conformation. The DNA unfolds after exposure to RNase or heat; this indicates that an RNA component of the complex is involved in stabilizing the structure.
摘要
大肠杆菌的DNA已被分离出来，其结构紧密，含有少量蛋白质和RNA，沉降系数约为3200 S。复合物中DNA的分子量非常大（可能高于10⁹）；蛋白质主要是核心RNA聚合酶；RNA主要是新生的信使核糖核酸链和核糖体链。含有该复合物的溶液粘度较低；这一点加上其沉降速率表明DNA处于紧密折叠的构象。DNA在暴露于核糖核酸酶或加热后会展开；这表明复合物中的RNA成分参与了结构的稳定。
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