Halbert S P, Bruderer R, Lin T M
J Exp Med. 1971 Apr 1;133(4):677-95. doi: 10.1084/jem.133.4.677.
When isolated beating ventricular heart cells from newborn rats were grown in tissue culture on untreated polystyrene surfaces, they showed a striking tendency to grow focally in three dimensions from the single layer cell sheets which were formed early in growth. During this process, they frequently formed miniature spherical heart-like masses, which continued to beat and grow in size. These often were somewhat lobulated in appearance, and grew up to 2 mm in diameter. Histological sections of such structures sometimes revealed evidence of appreciable orientation of the cells to each other, in fiber-like units. Electron microscope sections of such mini-hearts showed structures resembling intercalated discs between myocardial cells. The precise factors which induced the cardiac cells to apparently organize into these heart-like structures are not presently known.
当将新生大鼠分离的搏动性心室心肌细胞在未处理的聚苯乙烯表面进行组织培养时，它们呈现出一种显著的趋势，即从生长早期形成的单层细胞片开始在三维空间中局部生长。在此过程中，它们经常形成微型球形心脏样团块，这些团块继续搏动并长大。这些团块外观上常常有些分叶，直径可达2毫米。此类结构的组织学切片有时显示细胞彼此之间明显呈纤维状单位排列的证据。这种微型心脏的电子显微镜切片显示出类似于心肌细胞间闰盘的结构。目前尚不清楚诱导心肌细胞明显组织形成这些心脏样结构的确切因素。
