Haning R V, Levin R M, Behrman H R, Kase N G, Speroff L
Obstet Gynecol. 1979 Oct;54(4):442-7.
The plasma estradiol response is maximal 8--10 hours following mentropin injection. To obtain closer control, a menotropin protocol using 5 PM--8 PM injections and 8 AM blood sampling with a plasma estradiol window of 1000--2000 pg/ml was evaluated with simultaneous calibration of a urinary estriol glucuronide radioimmunoassay. One hundred twenty-eight paired urine and plasma samples were assayed in 48 cycles. In 26 cycles with paired samples on the day of human chorionic gonadotropin (hCG) injection, there were no cases of severe hyperstimulation, 2 cases of moderate hyperstimulation, and 11 pregnancies (42% of cycles given hCG). A window of between 40 and 100 micrograms/day of urinary estriol glucuronide corresponded to the 1000--2000 pg/ml plasma estradiol window by regression analysis. The pregnancy and hyperstimulation rates were compared with those observed in protocols previously published. Radioimmunoassay of urinary estriol glucuronide is faster and simpler than radioimmunoassay of plasma estradiol.
注射促卵泡素后8 - 10小时血浆雌二醇反应达到最大值。为了实现更精确的控制，评估了一种促卵泡素方案，该方案采用下午5点至8点注射，并在上午8点采集血样，血浆雌二醇窗口设定为1000 - 2000 pg/ml，同时对尿雌三醇葡萄糖醛酸苷放射免疫测定进行校准。在48个周期中对128对尿液和血浆样本进行了检测。在人绒毛膜促性腺激素（hCG）注射当天有配对样本的26个周期中，没有严重过度刺激的病例，有2例中度过度刺激，11例妊娠（占给予hCG周期的42%）。通过回归分析，尿雌三醇葡萄糖醛酸苷每天40至100微克的窗口对应于1000 - 2000 pg/ml的血浆雌二醇窗口。将妊娠率和过度刺激率与先前发表的方案中观察到的进行了比较。尿雌三醇葡萄糖醛酸苷的放射免疫测定比血浆雌二醇的放射免疫测定更快、更简单。
