Robrish S A, LeRoy A F, Chassy B M, Wilson J J, Krichevsky M I
Appl Microbiol. 1971 Feb;21(2):278-87. doi: 10.1128/am.21.2.278-287.1971.
This paper describes the properties and use of a fiber optic probe as an attachment to a spectrophotometer and its use for measurements in solutions and turbid suspensions. Measurements of a standard were identical when a spectrophotometer equipped with the probe was used or when a spectrophotometer was used in a conventional manner. The probe was calibrated for turbidimetric measurements with microorganisms by relating the apparent absorbancy measured on the spectrophotometer to the dry weight of each species of organism. Continuous measurements were made of the turbidity of growing cultures of Escherichia coli, Streptococcus mutans, and Saccharomyces cerevisiae. Transient changes in cell mass were observed in some cultures during continuous monitoring of growth. The data were recovered in a manner which allowed direct computer processing.
本文描述了一种光纤探头作为分光光度计附件的特性和用途，以及它在溶液和浑浊悬浮液测量中的应用。当使用配备该探头的分光光度计或采用传统方式使用分光光度计时，对标准品的测量结果是相同的。通过将分光光度计上测得的表观吸光度与每种生物体的干重相关联，对该探头进行了微生物比浊法测量的校准。对大肠杆菌、变形链球菌和酿酒酵母生长培养物的浊度进行了连续测量。在生长的连续监测过程中，在一些培养物中观察到了细胞质量的瞬时变化。数据以允许直接计算机处理的方式进行了回收。
