COULTAS M K, HUTCHISON D J
J Bacteriol. 1962 Sep;84(3):393-401. doi: 10.1128/jb.84.3.393-401.1962.
Coultas, M. Katharine (Sloan-Kettering Institute for Cancer Research, Rye, N.Y.) and Dorris J. Hutchison. Metabolism of resistant mutants of Streptococcus faecalis. IV. Use of a biophotometer in growth-curve studies. J. Bacteriol. 84:393-401. 1962. - Analysis of data obtained using the Bonet-Maury and Jouan biophotometer has shown it to be an accurate, automatic device for recording an entire bacterial growth curve, from which quantitative measurements of growth rate can be calculated. Growth rate values obtained in this manner agree with those obtained by conventional methods. The instrument is described in detail and reference is given to other uses of this type of photometer. The growth curves of Streptococcus faecalis ATCC 8043 and three mutants resistant to purine analogues were compared in media with and without a purine. Differences in these growth curves were evident. In the purine-deficient medium SF/AZAG, the 8-azaguanine-resistant mutant had the shortest doubling time, 61 min, followed by the two 6-mercaptopurine-resistant mutants (SF/MPcc, 72 min; SF/MP, 91 min), and finally the wild strain with a doubling time of 135 min. In the medium supplemented with xanthine, the respective doubling times were 52, 61, 68, and 68 min.
库尔塔斯，M. 凯瑟琳（纽约州莱伊市斯隆 - 凯特琳癌症研究所）和多里斯·J. 哈钦森。粪肠球菌抗性突变体的代谢。IV. 生物光度计在生长曲线研究中的应用。《细菌学杂志》84:393 - 401。1962年。——对使用博内 - 莫里和朱昂生物光度计获得的数据进行分析表明，它是一种准确的自动装置，可记录完整的细菌生长曲线，据此可计算生长速率的定量测量值。以这种方式获得的生长速率值与通过传统方法获得的值一致。详细描述了该仪器，并提及了这种类型光度计的其他用途。比较了粪肠球菌ATCC 8043和三种对嘌呤类似物有抗性的突变体在含嘌呤和不含嘌呤培养基中的生长曲线。这些生长曲线存在明显差异。在缺乏嘌呤的培养基SF/AZAG中，对8 - 氮杂鸟嘌呤有抗性的突变体倍增时间最短，为61分钟，其次是两种对6 - 巯基嘌呤有抗性的突变体（SF/MPcc，72分钟；SF/MP，91分钟），最后是野生菌株，倍增时间为135分钟。在添加了黄嘌呤的培养基中，各自的倍增时间分别为52、61、68和68分钟。
