Agayn V I, Walt D R
Department of Chemistry, Max Tishler Laboratory for Organic Chemistry, Tufts University, Medford, MA 02155.
Biotechnology (N Y). 1993 Jun;11(6):726-9. doi: 10.1038/nbt0693-726.
We have developed a fiber-optic chemical sensor for on-line monitoring of fermentation pH. The sensor is based on a covalently bound fluorescent dye immobilized within a water-permeable polymer layer on an optical fiber. Measurements were performed on a portable fluorimeter and employed a ratiometric approach to account for system instabilities. We show that the use of this fiber-optic sensor provides fast, accurate and reliable measurements during E. coli fermentation in a complex medium.
我们开发了一种用于在线监测发酵pH值的光纤化学传感器。该传感器基于一种共价结合的荧光染料，固定在光纤上的透水聚合物层内。测量是在便携式荧光计上进行的，并采用比率测量法来补偿系统不稳定性。我们表明，使用这种光纤传感器能够在复杂培养基中的大肠杆菌发酵过程中提供快速、准确和可靠的测量结果。
