Kornberg T, Gefter M L
Proc Natl Acad Sci U S A. 1971 Apr;68(4):761-4. doi: 10.1073/pnas.68.4.761.
The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+), and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A(1) (-) extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.
通过聚丙烯酰胺凝胶电泳判断，存在于Pol A(1) (-)大肠杆菌中的主要DNA合成酶（DNA聚合酶II）已被纯化至同质。该酶需要所有四种脱氧核苷三磷酸、Mg(++)、NH(4) (+)和天然DNA才能达到最大活性。该酶活性对巯基试剂敏感，对抗DNA聚合酶I抗血清不敏感。在Pol A(1) (-)提取物中已鉴定出第二种含量较低的DNA合成酶。讨论了这种酶与DNA聚合酶I和II的关系。
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